Immune Epitope Database (IEDB)

Reference
Reference Type	Literature	IEDB_Reference:1001817
Title	CD4 T helper type 1 and regulatory T cells induced against the same epitopes on the core protein in hepatitis C virus-infected persons.
Authors	Angus J MacDonald; Margaret Duffy; Miriam T Brady; Susan McKiernan; William Hall; John Hegarty; Michael Curry; Kingston H G Mills
Affiliations	Immune Regulation Research Group, Department of Biochemistry, Trinity College Dublin, Ireland.
Journal	J Infect Dis	PMID:11920289
Year	2002
Abstract	The factors that determine persistence or clearance of hepatitis C virus (HCV) infection are poorly understood. The CD4 T cell responses to the HCV core protein were examined in a cohort of women infected with a single genotype of HCV. CD4 T cells from HCV-infected patients secreted interferon (IFN)-gamma in response to peptides from 4 immunodominant regions of the core protein, and these responses were stronger in persistently infected women. Interleukin (IL)-10 was also produced by CD4 T cells from HCV-infected subjects in response to the same core peptides. Furthermore, HCV core-specific CD4 T cell clones secreted either IFN-gamma or IL-10 but not IL-4. These findings demonstrate that T helper type 1 and regulatory T cells are induced against the same epitopes on the core protein during HCV infection.
Curation Last Updated	2023-08-18 20:11:58

Epitope
Epitope ID	717	IEDB_epitope:717
Chemical Type	Linear peptide
Linear Sequence	ADLMGYIPLVGAPLGGAARA
Source Molecule Name	polyprotein	GenPept:BAA03375.1
Source Organism	Hepatitis C virus subtype 1b (Hepatitis C virus type 1b)	NCBITaxon:31647
Starting Position	131
Ending Position	150

Epitope Reference Details
Epitope Structure Defines	Epitope containing region/antigenic site
Epitope Name	Core 131-150
Reference Starting Position	131
Reference Ending Position	150
Location of Data in Reference	Figure 1

Immunization
Host Organism	Homo sapiens (human)	NCBITaxon:9606

Host Details
Sex	F
Age	41-55
MHC Types present	HLA-DRB107:01;HLA-DQB105:01;HLA-DRB101:01;HLA-DQB102:01

1st In Vivo Process
In Vivo Process Type	Occurrence of infectious disease	ONTIE:0003317
Disease State	hepatitis C	DOID:1883
Disease Stage	Chronic;	OGMS:0000064

1st Immunogen
Epitope Relation	Source Organism
Object Type	Organism
Organism	Hepatitis C virus subtype 1b (Hepatitis C virus type 1b)	NCBITaxon:31647

Immunogen Details
Immunogen Reference Name	Hepatitis C virus subtype 1b

Immunization Comments
Immunization Comments	PBMC were harvested from a group of Irish women infected with HCV 1b through the administration of contaminated anti-D Ig.

T Cell Assay
Qualitative Measurement	Positive
Method/Technique	ELISA	OBI:1110151
Measurement of	IFNg release

Effector Cells
Effector Cell Tissue Type	Blood	UBERON:0000178
Effector Cell Type	PBMC	CL:2000001
Effector Cell Culture Conditions	Direct Ex Vivo

Antigen Presenting Cells
Cell Tissue Type	Blood	UBERON:0000178
Cell Type	PBMC	CL:2000001
Cell Culture Conditions	Direct Ex Vivo

Antigen
Epitope Relation	Epitope
Chemical Type	Linear peptide
Linear Sequence	ADLMGYIPLVGAPLGGAARA
Source Molecule Name	polyprotein	GenPept:BAA03375.1
Source Organism	Hepatitis C virus subtype 1b (Hepatitis C virus type 1b)	NCBITaxon:31647
Starting Position	131
Ending Position	150

Antigen Details
Antigen Reference Name	Core 131-150

Assay Reference Details
Assay Comments by IEDB Curator	PBMC from HCV infected patients produced IFNg in response to the epitope. Chronically infected patients (HCV PCR+) responded more often than patients who cleared the virus (HCV RNA-).
Location of Assay Data in Reference	Figure 2 and table 1