Reference
Reference TypeLiterature
IEDB_Reference:1009746
TitleIdentification of T- and B-cell epitopes of the S2 and S3 subunits of pertussis toxin by use of synthetic peptides.
AuthorsP Chong; G Zobrist; C Sia; S Loosmore; M Klein
AffiliationsConnaught Centre for Biotechnology Research, Connaught Laboratories Ltd., Willowdale, Ontario, Canada.
JournalInfect Immun
PMID:1383153
Year1992
AbstractTo design an optimized synthetic vaccine against whooping cough, we have studied the biological and immunological properties of three peptides of the S2 subunit and nine overlapping synthetic peptides covering the entire sequence of the S3 subunit of pertussis toxin (PT). Synthetic peptides corresponding to sequences 18 to 41, 78 to 108, 134 to 154, and 149 to 176 of S3 were found to be consistently capable of stimulating the proliferation of PT-specific T-cell lines primed with pertussis toxoid in both BALB/c and A/J strains of mice. All synthetic peptides were recognized by rabbit antisera raised against PT or pertussis toxoid. Both S2 and S3 peptide-keyhole limpet hemocyanin (KLH) conjugates in the presence of complete Freund's adjuvant induced peptide-specific antibody responses in rabbits, and the antisera raised against S2(1-23), S3(18-41), S3(37-64), and S3(149-176) peptide-KLH conjugates cross-reacted with both subunits in the immunoblots. All antisera except those against S2(123-154) and S3(103-127) reacted with native PT in an enzyme-linked immunosorbent assay (ELISA) with PT directly coated onto microtiter wells. In contrast, antisera raised against S2(123-154), S3(1-23), S3(18-41), S3(37-64), S3(60-87), and S3(103-127) peptide-KLH conjugates recognized native PT in a fetuin-PT capture ELISA. S2(78-98), S3(1-23), and S3(149-176) peptide-KLH conjugates elicited good PT-neutralizing antibody responses as judged by the antitoxin CHO cell assay. Identification of these B-cell neutralization epitopes and T-cell immunodominant determinants represents a first step towards the rational design of a synthetic vaccine against whooping cough.
Curation Last Updated2023-08-18 20:32:51
Epitope
Epitope ID1508
IEDB_epitope:1508
Chemical TypeLinear peptide
Linear SequenceAGFIYRETFCITTIYKTGQPAADHYYSKVTA
Source Molecule NamePertussis toxin subunit 3 precursor
GenPept:P04979.1
Source OrganismBordetella pertussis
NCBITaxon:520
Starting Position106
Ending Position136
Epitope Reference Details
Epitope Structure DefinesEpitope containing region/antigenic site
Epitope NamePT S3(78-108)
Location of Data in ReferenceTable 1
Immunization
Host OrganismMus musculus BALB/c
ONTIE:0000001
1st In Vivo Process
In Vivo Process TypeAdministration in vivo
OBI:0600007
Administration Details
Dose Schedule1 doses of 5ug.
1st Immunogen
Epitope RelationSource Antigen
Chemical TypeProtein
Molecule NamePertussis toxin subunit 3 precursor
GenPept:P04979.1
OrganismBordetella pertussis
NCBITaxon:520
Immunization Comments
Immunization CommentsMice were primed with 5ug of pertussis toxoid in A1P04.
T Cell Assay
Qualitative MeasurementPositive-High
Method/Technique3H-thymidine
OBI:1110180
Measurement ofproliferation
Effector Cells
Effector Cell Tissue TypeSpleen
UBERON:0002106
Effector Cell TypeSplenocyte
CL:2000074
Effector Cell Culture ConditionsDirect Ex Vivo
Antigen Presenting Cells
Cell Tissue TypeSpleen
UBERON:0002106
Cell TypeSplenocyte
CL:2000074
Cell Culture ConditionsDirect Ex Vivo
MHC Allele
MHC Allele NameH2-d class II
MRO:0001774
MHC Evidence CodeT cell assay -Biological process measured
ECO:0008083
Antigen
Epitope RelationEpitope
Chemical TypeLinear peptide
Linear SequenceAGFIYRETFCITTIYKTGQPAADHYYSKVTA
Source Molecule NamePertussis toxin subunit 3 precursor
GenPept:P04979.1
Source OrganismBordetella pertussis
NCBITaxon:520
Starting Position106
Ending Position136
Assay Reference Details
Location of Assay Data in ReferenceFigure 3B