| Reference | ||
|---|---|---|
| Reference Type | Literature | IEDB_Reference:1006618 |
| Title | Delayed infection after immunization with a peptide from the transmembrane glycoprotein of the feline immunodeficiency virus. | |
| Authors | J Richardson; A Moraillon; F Crespeau; S Baud; P Sonigo; G Pancino | |
| Affiliations | Génétique des Virus (ICGM-CNRS UPR 0415), Institut Cochin de Génétique Moléculaire, Paris, France. | |
| Journal | J Virol | |
| Year | 1998 | |
| Abstract | Recent advances in the quantitative assessment of viral burden, by permitting the extension of criteria applied to assess the efficacy of vaccines from all-or-none protection to diminution of the viral burden, may allow the identification of original immunogens of value in combined vaccines. Peptides corresponding to three domains of the envelope glycoproteins of feline immunodeficiency virus that are recognized during natural infection were used to immunize cats. After challenge with a primary isolate of feline immunodeficiency virus, the development of acute infection was monitored by quantitative assessment of the viral burden in plasma and tissues by competitive reverse transcription-PCR, by measurement of the humoral response developed to viral components, and by lymphocyte subset analysis. Whereas immunization with two peptides derived from the surface glycoprotein had no effect on the early course of infection, immunization with a peptide derived from the transmembrane glycoprotein delayed infection, as reflected by a diminished viral burden in the early phase of primary infection and delayed seroconversion. This peptide, located in the membrane-proximal region of the extracellular domain, has homology to an epitope of human immunodeficiency virus type 1 recognized by a broadly neutralizing monoclonal antibody. These results suggest that lentivirus transmembrane glycoproteins share a determinant in the juxtamembrane ectodomain which could be of importance in the design of vaccines against AIDS. | |
| Curation Last Updated | 2023-08-18 20:27:50 | |
| Epitope | ||
|---|---|---|
| Epitope ID | 87755 | IEDB_epitope:87755 |
| Chemical Type | Linear peptide | |
| Linear Sequence | QVVKQPDYLVVPGEVMEYKPRR | |
| Source Molecule Name | Envelope glycoprotein gp150 | |
| Source Organism | Feline immunodeficiency virus (isolate wo) | |
| Starting Position | 586 | |
| Ending Position | 607 | |
| Epitope Reference Details | ||
|---|---|---|
| Epitope Structure Defines | Epitope containing region/antigenic site | |
| Epitope Name | SU5 | |
| Reference Starting Position | 586 | |
| Reference Ending Position | 607 | |
| Location of Data in Reference | Materials and Methods | |
| Immunization | ||
|---|---|---|
| Host Organism | Felis catus (cat) | |
| Host Details | ||
|---|---|---|
| Age | 4 month old | |
| 1st In Vivo Process | ||
|---|---|---|
| In Vivo Process Type | Administration in vivo to prevent or reduce disease | |
| 1st In Vivo Process Administration Details | ||
|---|---|---|
| Adjuvants | Freund's complete; | VO:0000139 |
| Route | subcutaneous | |
| Dose Schedule | 5 doses of 120 μg of FIV peptide (approximately 250 μg of KLH-coupled peptide) | |
| 1st Immunogen | ||
|---|---|---|
| Epitope Relation | Epitope | |
| Chemical Type | Linear peptide | |
| Linear Sequence | QVVKQPDYLVVPGEVMEYKPRR | |
| Source Molecule Name | Envelope glycoprotein gp150 | |
| Source Organism | Feline immunodeficiency virus (isolate wo) | |
| Starting Position | 586 | |
| Ending Position | 607 | |
| Immunization Comments | ||
|---|---|---|
| Immunization Comments | Immunogen carrier: Peptide conjugate: Keyhole limpet hemocyanin (Megathura crenulata). Four booster injections were administered subcutaneously in incomplete Freund´s adjuvant, at intervals of 2 to 3 weeks for the 1st, 2nd and 3rd, and 7 weeks for the 4th. A cysteine residue was added at the N-terminus of the peptide for coupling to KLH. | |
| B Cell Assay | ||
|---|---|---|
| Qualitative Measurement | Positive-Low | |
| Method/Technique | in vivo assay | |
| Measurement of | protection from pathogen challenge | |
| Measurement Details | ||
|---|---|---|
| Number of Subjects Tested | 4 | |
| Number of Subjects Responded | 1 | |
| Response Frequency (%) | 25 | |
| Antigen | ||
|---|---|---|
| Epitope Relation | Source Organism | |
| Object Type | Organism | |
| Organism | Feline immunodeficiency virus (isolate wo) | |
| Assay Reference Details | ||
|---|---|---|
| Assay Comments by IEDB Curator | Cats were challenged 1 week after the final immunization by intraperitoneal inoculation of 10 50% cat infectious doses (CID50) of the Wo isolate of FIV. The kinetics of appearance of viral RNA in plasma were delayed in one cat compared to control cats. Levels of viral RNA in axillary lymph nodes, spleen and thymus were similar to those in control cats. | |
| Location of Assay Data in Reference | Figures 3 and 4 | |