Reference
Reference TypeLiterature
IEDB_Reference:1039261
TitleImmunoreactive peptide maps of SARS-CoV-2.
AuthorsNischay Mishra; Xi Huang; Shreyas Joshi; Cheng Guo; James Ng; Riddhi Thakkar; Yongjian Wu; Xin Dong; Qianlin Li; Richard S Pinapati; Eric Sullivan; Adrian Caciula; Rafal Tokarz; Thomas Briese; Jiahai Lu; W Ian Lipkin
AffiliationsCenter for Infection and Immunity, Mailman School of Public Health, Columbia University, New York, NY, USA. nm2641@cumc.columbia.edu; Sun Yat-sen University, Guangzhou, Guangdong Province, China; Center for Infection and Immunity, Fifth Affiliated Hospital, Sun Yat-sen University, Zhuhai, Guangdong Province, China; School of Public Health, Sun Yat-sen University, Guangzhou, Guangdong Province, China; Nimble Therapeutics Inc, Madison, WI, USA; Sun Yat-sen University, Guangzhou, Guangdong Province, China. lujiahai@mail.sysu.edu.cn; School of Public Health, Sun Yat-sen University, Guangzhou, Guangdong Province, China. lujiahai@mail.sysu.edu.cn; Center for Infection and Immunity, Mailman School of Public Health, Columbia University, New York, NY, USA. wil2001@cumc.columbia.edu.
JournalCommun Biol
PMID:33580175
Year2021
AbstractSerodiagnosis of SARS-CoV-2 infection is impeded by immunological cross-reactivity among the human coronaviruses (HCoVs): SARS-CoV-2, SARS-CoV-1, MERS-CoV, OC43, 229E, HKU1, and NL63. Here we report the identification of humoral immune responses to SARS-CoV-2 peptides that may enable discrimination between exposure to SARS-CoV-2 and other HCoVs. We used a high-density peptide microarray and plasma samples collected at two time points from 50 subjects with SARS-CoV-2 infection confirmed by qPCR, samples collected in 2004-2005 from 11 subjects with IgG antibodies to SARS-CoV-1, 11 subjects with IgG antibodies to other seasonal human coronaviruses (HCoV), and 10 healthy human subjects. Through statistical modeling with linear regression and multidimensional scaling we identified specific peptides that were reassembled to identify 29 linear SARS-CoV-2 epitopes that were immunoreactive with plasma from individuals who had asymptomatic, mild or severe SARS-CoV-2 infections. Larger studies will be required to determine whether these peptides may be useful in serodiagnostics.
Curation Last Updated2025-03-25 21:53:15
Epitope
Epitope ID1528356
IEDB_epitope:1528356
Chemical TypeLinear peptide
Linear SequenceVRDKGALEKFMS
Source Molecule NameORF1ab [Middle East respiratory syndrome-related coronavirus]
GenPept:AVV62535.1
Source OrganismMiddle East respiratory syndrome-related coronavirus (MERS coronavirus)
NCBITaxon:1335626
Starting Position6580
Ending Position6591
Epitope Reference Details
Epitope Structure DefinesEpitope containing region/antigenic site
Epitope NameVRDKGALEKFMS
Location of Data in ReferenceSupplementary Data 1
Immunization
Host OrganismHomo sapiens (human)
NCBITaxon:9606
Host Details
Host GeolocationChina
GAZ:00002845
1st In Vivo Process
In Vivo Process TypeOccurrence of infectious disease
ONTIE:0003317
Disease StateCOVID-19
DOID:0080600
Disease StageAcute/Recent onset;OGMS:0000094
1st Immunogen
Epitope RelationTaxonomic Sibling
Object TypeOrganism
OrganismSARS-CoV2
NCBITaxon:2697049
B Cell Assay
Qualitative MeasurementNegative
Method/Techniquemicroarray
OBI:0002053
Measurement ofqualitative binding
Assayed Antibody
Assayed Antibody Source MaterialSerum
UBERON:0001977
Assayed Antibody Immunoglobulin DomainEntire Antibody
Assayed Antibody Purification StatusPolyclonal
Assayed Antibody Heavy Chain TypeIgM
Antigen
Epitope RelationEpitope
Chemical TypeLinear peptide
Linear SequenceVRDKGALEKFMS
Source Molecule NameORF1ab [Middle East respiratory syndrome-related coronavirus]
GenPept:AVV62535.1
Source OrganismMiddle East respiratory syndrome-related coronavirus (MERS coronavirus)
NCBITaxon:1335626
Starting Position6580
Ending Position6591
Assay Reference Details
Location of Assay Data in ReferenceSupplementary Data 2