| Reference |
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| Reference Type | Literature | IEDB_Reference:1045727 |
| Title | Large-Scale Screening of CD4+ T-Cell Epitopes From SARS-CoV-2 Proteins and the Universal Detection of SARS-CoV-2 Specific T Cells for Northeast Asian Population. | |
| Authors | Yu Zhao; Chengtao He; Min Peng; Min Li; Xiaotao Liu; Xuelian Han; Qiang Fu; Yandan Wu; Fangping Yue; Chunguang Yan; Guangyu Zhao; Chuanlai Shen | |
| Affiliations | Department of Microbiology and Immunology, Medical School of Southeast University, Nanjing, China; Nanjing Red Cross Blood Center, Nanjing, China; State Key Laboratory of Pathogen and Biosecurity, Academy of Military Medical Sciences, Beijing, China; Laboratory of Advanced Biotechnology, Academy of Military Medical Sciences, Beijing, China. | |
| Journal | J Med Virol | |
| Year | 2025 | |
| Abstract | The polymorphism of human leukocyte antigens in the Northeast Asian populations and the lack of broad-spectrum T-cell epitopes covering this cohort markedly limited the development of T cell-directed vaccines against SARS-CoV-2 infection, and also hampered the universal detection of SARS-CoV-2 specific T cells. In this study, 93 CD4<sup>+</sup> T-cell epitopes restricted by 12 prevalent HLA-DRB1 allotypes, which covering over 80% Chinese and Northeast Asian populations, were identified from the S, E, M, N and RdRp proteins of SARS-CoV-2 by in silico prediction, DC-peptide-PBL coculture experiment, and immunization in HLA-A2/DR1 transgenic mice. Furthermore, by using validated 215 CD8<sup>+</sup> T cell epitope peptides and 123 CD4<sup>+</sup> T-cell epitope peptides covering Northeast Asian cohort, the universal ELISpot detection systems of SARS-CoV-2 specific CD8<sup>+</sup> T cells and CD4<sup>+</sup> T cells were established, for the first time, and followed by the tests for 50 unexposed and 100 convalescent samples. The median of spot-forming units for CD8<sup>+</sup> T cells and CD4<sup>+</sup> T cells were 68 and 15, respectively, in the unexposed donors, but were 137 and 52 in the convalescent donors 6 months after recovery while 128 and 47 in the convalescent donors 18 months after recovery. This work initially provided the broad-spectrum CD4<sup>+</sup> T-cell epitope library of SARS-CoV-2 for the design of T cell-directed vaccines and the universal T cell detection tool tailoring to Northeast Asian population, and confirmed the long-term memory T cell immunity after SARS-CoV-2 infection. | |
| Curation Last Updated | 2025-03-11 20:01:05 | |