| Reference | ||
|---|---|---|
| Reference Type | Literature | IEDB_Reference:1032598 |
| Title | Assessment of Antipiperacillin IgG Binding to Structurally Related Drug Protein Adducts. | |
| Authors | Mohammed O Amali; Rosalind E Jenkins; Xiaoli Meng; Lee Faulkner; Paul Whitaker; Daniel Peckham; B Kevin Park; Dean J Naisbitt | |
| Affiliations | Department of Pharmacology, University of Liverpool , Sherrington Building, Ashton Street, Liverpool L69 3GE, England; Regional Adult Cystic Fibrosis Unit, St. James's Hospital , Leeds LS9 7TF, England. | |
| Journal | Chem Res Toxicol | |
| Year | 2017 | |
| Abstract | The risk of developing hypersensitivity to alternative antibiotics is a concern for penicillin hypersensitive patients and healthcare providers. Herein we use piperacillin hypersensitivity as a model to explore the reactivity of drug-specific IgG against alternative -lactam protein adducts. Mass spectrometry was used to show the drugs (amoxicillin, flucloxacillin, benzyl penicillin, aztreonam, and piperacillin) bind to similar lysine residues on the protein carrier bovine serum albumin. However, the hapten-specific IgG antibodies found in piperacillin hypersensitive patient plasma did not bind to other -lactam protein conjugates. These data outline the fine specificity of piperacillin-specific IgG antibodies that circulate in patients with hypersensitivity. | |
| Curation Last Updated | 2025-04-11 18:53:59 | |
| Epitope | ||
|---|---|---|
| Epitope ID | 742523 | IEDB_epitope:742523 |
| Chemical Type | Non-peptidic | |
| Smiles Structure | N(C(=O)C=1C(=NOC1C)C2=C(C=CC=C2F)Cl)[C@@H]([C@@]3(N[C@H](C(S3)(C)C)C(O)=O)[H])C(=O)NCCCC[C@@H](C(=O)O)N | |
| Molecule Name | flucloxacilloyl-L-lysine | |
| Epitope Reference Details | ||
|---|---|---|
| Epitope Structure Defines | Partial Epitope | |
| Epitope Name | flucloxacillin-lysine | |
| Location of Data in Reference | Figure 1 | |
| Immunization | ||
|---|---|---|
| Host Organism | Homo sapiens (human) | |
| Host Details | ||
|---|---|---|
| Host Geolocation | England | |
| 1st In Vivo Process | ||
|---|---|---|
| In Vivo Process Type | Occurrence of allergy | |
| Disease State | piperacillin allergy | |
| Disease Stage | Chronic; | OGMS:0000064 |
| 1st Immunogen | ||
|---|---|---|
| Epitope Relation | Structurally Related | |
| Chemical Type | Non-peptidic | |
| Smiles Structure | [H][C@]12SC(C)(C)[C@@H](N1C(=O)[C@H]2NC(=O)[C@H](NC(=O)N1CCN(CC)C(=O)C1=O)c1ccccc1)C(O)=O | |
| Molecule Name | piperacillin | |
| Immunization Comments | ||
|---|---|---|
| Immunization Comments | Plasma from four hypersensitive patients who developed a maculopapular rash after piperacillin therapy was tested. | |
| B Cell Assay | ||
|---|---|---|
| Qualitative Measurement | Positive-Low | |
| Method/Technique | ELISA | |
| Measurement of | qualitative binding | |
| Measurement Details | ||
|---|---|---|
| Number of Subjects Tested | 4 | |
| Assayed Antibody | ||
|---|---|---|
| Assayed Antibody Source Material | Plasma | |
| Assayed Antibody Immunoglobulin Domain | Entire Antibody | |
| Assayed Antibody Purification Status | Polyclonal | |
| Assayed Antibody Heavy Chain Type | IgG | |
| Antigen | ||
|---|---|---|
| Epitope Relation | Epitope | |
| Chemical Type | Non-peptidic | |
| Smiles Structure | N(C(=O)C=1C(=NOC1C)C2=C(C=CC=C2F)Cl)[C@@H]([C@@]3(N[C@H](C(S3)(C)C)C(O)=O)[H])C(=O)NCCCC[C@@H](C(=O)O)N | |
| Molecule Name | flucloxacilloyl-L-lysine | |
| Assay Reference Details | ||
|---|---|---|
| Assay Comments by IEDB Curator | Antigen carrier: Protein conjugate: Albumin (Bos taurus (bovine)). | |
| Location of Assay Data in Reference | Figure 3 | |