Reference
Reference TypeLiterature
TitlePeptide mapping of bovine T-cell epitopes for the 38 kDa tuberculosis antigen.
AuthorsJ M Pollock; A J Douglas; D P Mackie; S D Neill
AffiliationsDepartment of Agriculture for Northern Ireland, Stormont, Belfast, UK.
JournalScand J Immunol
Year1995
AbstractMycobacterium bovis infection in cattle continues to be a problem in several regions, partly due to inadequate diagnostic tests. The aim of this study was to use an experimental model of the natural disease to identify T-cell epitopes from the mycobacterial 38 kDa antigen as potentially specific diagnostic reagents. A panel of overlapping synthetic peptides (16-mers with a five-residue overlap) were produced from the published amino acid sequence. It was found that peripheral blood lymphocytes from at least three of four experimentally infected animals, which were considered to be in either Th1- or Th1/Th2-dominated stages of anti-mycobacterial immunity, proliferated in response to five epitopes (residues 1-27, 88-107, 122-138, 243-260 and 307-328). However, in vitro production of IFN-gamma was detected only in response to epitope 122-138, indicating a role in protective immunity. The peptides were not recognized by control, uninfected animals, but all epitopes showed various degrees of recognition by animals which were field reactors to intradermal tuberculin testing. Furthermore, epitopes 1-27, 88-107 and 122-138 were recognized by four breeds of cattle and by animals from separate herds, suggesting genetic permissiveness in recognition which would be essential in the development of a diagnostic test.
Curation Last Updated2023-08-18 20:09:38
Epitope
Epitope ID46997
Chemical TypeLinear peptide
Linear SequencePAVVKLSDALIATISS
Source Molecule NamePhosphate-binding protein pstS 1 precursor
Source OrganismMycobacterium tuberculosis
Starting Position359
Ending Position374
Epitope Reference Details
Epitope Structure DefinesEpitope containing region/antigenic site
Epitope NamePeptide 34
Reference Starting Position364
Reference Ending Position379
Location of Data in ReferenceReference 24
Immunization
Host OrganismBos taurus (bovine)
1st In Vivo Process
In Vivo Process TypeAdministration in vivo
Administration Details
Routeintranasal mucosal
Dose Schedule1
1st Immunogen
Epitope RelationTaxonomic Sibling
Object TypeOrganism
OrganismMycobacterium bovis T/91/1378
Immunogen Details
Immunogen Reference NameMycobacterium bovis
Immunization Comments
Immunization CommentsFour cows were immunized. Two cows were directly immunized intranasally with 107 colony-forming units of strain T/91/1378 of Mycobacterium bovis isolated from a field case of bovine tuberculosis. Two cows were contact infected by being introduced to the same enclosed airspace as the directly immunized cows at 28 weeks post-immunization. All four animals showed proliferative responses to mycobacterial purified protein derivative.
T Cell Assay
Qualitative MeasurementPositive
Method/Technique3H-thymidine
Measurement ofproliferation
Measurement Details
Number of Subjects Tested4
Number of Subjects Responded1
Response Frequency (%)25
Effector Cells
Effector Cell Tissue TypeBlood
Effector Cell TypePBMC
Effector Cell Culture ConditionsDirect Ex Vivo
Antigen Presenting Cells
Cell Tissue TypeBlood
Cell TypePBMC
Cell Culture ConditionsDirect Ex Vivo
MHC Allele
MHC Allele NameBoLA class II
MHC Evidence CodeT cell assay -Biological process measured
Antigen
Epitope RelationEpitope
Chemical TypeLinear peptide
Linear SequencePAVVKLSDALIATISS
Source Molecule NamePhosphate-binding protein pstS 1 precursor
Source OrganismMycobacterium tuberculosis
Starting Position359
Ending Position374
Antigen Details
Antigen Reference NamePeptide 34
Assay Reference Details
Assay Comments by IEDB CuratorFour cows that had been exposed to Mycobacterium bovis were screened for T cell proliferative responses to an overlapping 16-mer peptide library encompassing the entire 38 kDa protein from Mycobacterium tuberculosis. All four animals showed proliferative responses to mycobacterial purified protein derivative. One of the four animals showed proliferative responses to the epitope, but none produced detectable levels of IFNγ in response to the epitope in a cytokine ELISA assay.
Location of Assay Data in ReferenceFigure 2