Reference | ||
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Reference Type | Literature | IEDB_Reference:1001034 |
Title | Peptide mapping of bovine T-cell epitopes for the 38 kDa tuberculosis antigen. | |
Authors | J M Pollock; A J Douglas; D P Mackie; S D Neill | |
Affiliations | Department of Agriculture for Northern Ireland, Stormont, Belfast, UK. | |
Journal | Scand J Immunol | |
Year | 1995 | |
Abstract | Mycobacterium bovis infection in cattle continues to be a problem in several regions, partly due to inadequate diagnostic tests. The aim of this study was to use an experimental model of the natural disease to identify T-cell epitopes from the mycobacterial 38 kDa antigen as potentially specific diagnostic reagents. A panel of overlapping synthetic peptides (16-mers with a five-residue overlap) were produced from the published amino acid sequence. It was found that peripheral blood lymphocytes from at least three of four experimentally infected animals, which were considered to be in either Th1- or Th1/Th2-dominated stages of anti-mycobacterial immunity, proliferated in response to five epitopes (residues 1-27, 88-107, 122-138, 243-260 and 307-328). However, in vitro production of IFN-gamma was detected only in response to epitope 122-138, indicating a role in protective immunity. The peptides were not recognized by control, uninfected animals, but all epitopes showed various degrees of recognition by animals which were field reactors to intradermal tuberculin testing. Furthermore, epitopes 1-27, 88-107 and 122-138 were recognized by four breeds of cattle and by animals from separate herds, suggesting genetic permissiveness in recognition which would be essential in the development of a diagnostic test. | |
Curation Last Updated | 2023-08-18 20:09:38 |
Epitope | ||
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Epitope ID | 46997 | IEDB_epitope:46997 |
Chemical Type | Linear peptide | |
Linear Sequence | PAVVKLSDALIATISS | |
Source Molecule Name | Phosphate-binding protein pstS 1 precursor | |
Source Organism | Mycobacterium tuberculosis | |
Starting Position | 359 | |
Ending Position | 374 |
Epitope Reference Details | ||
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Epitope Structure Defines | Epitope containing region/antigenic site | |
Epitope Name | Peptide 34 | |
Reference Starting Position | 364 | |
Reference Ending Position | 379 | |
Location of Data in Reference | Reference 24 |
Immunization | ||
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Host Organism | Bos taurus (bovine) |
1st In Vivo Process | ||
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In Vivo Process Type | Administration in vivo |
Administration Details | ||
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Route | intranasal mucosal | |
Dose Schedule | 1 |
1st Immunogen | ||
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Epitope Relation | Taxonomic Sibling | |
Object Type | Organism | |
Organism | Mycobacterium bovis T/91/1378 |
Immunogen Details | ||
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Immunogen Reference Name | Mycobacterium bovis |
Immunization Comments | ||
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Immunization Comments | Four cows were immunized. Two cows were directly immunized intranasally with 107 colony-forming units of strain T/91/1378 of Mycobacterium bovis isolated from a field case of bovine tuberculosis. Two cows were contact infected by being introduced to the same enclosed airspace as the directly immunized cows at 28 weeks post-immunization. All four animals showed proliferative responses to mycobacterial purified protein derivative. |
T Cell Assay | ||
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Qualitative Measurement | Positive | |
Method/Technique | 3H-thymidine | |
Measurement of | proliferation |
Measurement Details | ||
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Number of Subjects Tested | 4 | |
Number of Subjects Responded | 1 | |
Response Frequency (%) | 25 |
Effector Cells | ||
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Effector Cell Tissue Type | Blood | |
Effector Cell Type | PBMC | |
Effector Cell Culture Conditions | Direct Ex Vivo |
Antigen Presenting Cells | ||
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Cell Tissue Type | Blood | |
Cell Type | PBMC | |
Cell Culture Conditions | Direct Ex Vivo |
MHC Allele | ||
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MHC Allele Name | BoLA class II | |
MHC Evidence Code | T cell assay -Biological process measured |
Antigen | ||
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Epitope Relation | Epitope | |
Chemical Type | Linear peptide | |
Linear Sequence | PAVVKLSDALIATISS | |
Source Molecule Name | Phosphate-binding protein pstS 1 precursor | |
Source Organism | Mycobacterium tuberculosis | |
Starting Position | 359 | |
Ending Position | 374 |
Antigen Details | ||
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Antigen Reference Name | Peptide 34 |
Assay Reference Details | ||
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Assay Comments by IEDB Curator | Four cows that had been exposed to Mycobacterium bovis were screened for T cell proliferative responses to an overlapping 16-mer peptide library encompassing the entire 38 kDa protein from Mycobacterium tuberculosis. All four animals showed proliferative responses to mycobacterial purified protein derivative. One of the four animals showed proliferative responses to the epitope, but none produced detectable levels of IFNγ in response to the epitope in a cytokine ELISA assay. | |
Location of Assay Data in Reference | Figure 2 |