Reference
Reference TypeLiterature
TitleIdentification of T cell stimulatory epitopes from the 18 kDa protein of Mycobacterium leprae.
AuthorsT M Doherty; B T Bäckström; S G Love; D R Harding; J D Watson
AffiliationsDepartment of Molecular Medicine, University of Auckland School of Medicine, New Zealand.
JournalInt Immunol
Year1993
AbstractWe have used different mouse strains to examine in vivo and in vitro responses to the 18 kDa protein of Mycobacterium leprae, which appears to be strongly immunogenic in both mice and humans. B and T cell stimulatory epitopes recognised by different strains of mice have been mapped using overlapping peptides that span the entire 18 kDa protein. Previous work established that immunization of mice with the 18 kDa protein results in specific antibody production to common B cell epitopes and immunization of mice with peptides containing these B cell epitopes resulted in the induction of specific IgG to only a limited subset of epitopes in each strain. Now we report that T cells purified from mice immunized with peptides that stimulate antibody production, proliferate in vitro when rechallenged. The proliferating T cells produce levels of IL-2 and IFN-gamma, that indicate antigen-specific T helper type 1 cells are present in significant numbers. Thus, a comparison of in vivo and in vitro data suggests that T cells bearing the phenotype associated with potentially protective cell-mediated responses can be primed in vivo by epitopes on small peptides. Since T cells from both strains of mice are capable of responding to the immunogenic synthetic peptides in vitro, but give different responses to the same peptides in vivo, factors other than epitope structure appear to influence T cell subset activation. This may have important implications for diseases such as leprosy where a polarized T cell response appears to develop and for the development of synthetic subunit vaccines.
Curation Last Updated2023-11-21 22:20:51
Epitope
Epitope ID13941
Chemical TypeLinear peptide
Linear SequenceEQVLGTSARPAVMPMDAWRE
Source Molecule Name18 kDa antigen
Source OrganismMycobacterium leprae
Starting Position16
Ending Position35
Epitope Reference Details
Epitope Structure DefinesEpitope containing region/antigenic site
Epitope NameM. leprae 18 kDa protein amino acid residues 16-35
Reference Starting Position16
Reference Ending Position35
Location of Data in ReferenceFigure 3
Immunization
Host OrganismMus musculus B10.BR
Host Details
Age6-10 weeks
MHC Types presentH2-k class I;H2-k class II
1st In Vivo Process
In Vivo Process TypeAdministration in vivo
Administration Details
AdjuvantsFreund's incomplete (IFA);VO:0000142
Routesubcutaneous
1st Immunogen
Epitope RelationSource Antigen
Chemical TypeProtein
Molecule Name18 kDa antigen
OrganismMycobacterium leprae
Immunogen Details
Immunogen Reference Name18 kDa antigen
Immunization Comments
Immunization CommentsAntigen was dissolved in PBS then emulsified in IFA (50:50 v/v) then given via the hind footpads (25 ul each) and at the base of the tail (50 ul). T cells were prepared 7-14 days after the final immunization.
T Cell Assay
Qualitative MeasurementPositive
Method/Technique3H-thymidine
Measurement ofproliferation
Effector Cells
Effector Cell Tissue TypeLymphoid
Effector Cell TypeT cell
Effector Cell Culture ConditionsDirect Ex Vivo
Antigen Presenting Cells
Cell Tissue TypeBone Marrow
Cell TypeMacrophage
Cell Culture ConditionsDirect Ex Vivo
MHC Allele
MHC Allele NameH2-k class II
MHC Evidence CodeT cell assay -Biological process measured
Antigen
Epitope RelationEpitope
Chemical TypeLinear peptide
Linear SequenceEQVLGTSARPAVMPMDAWRE
Source Molecule Name18 kDa antigen
Source OrganismMycobacterium leprae
Starting Position16
Ending Position35
Antigen Details
Antigen Reference NameM. leprae 18 kDa protein amino acid residues 16-35
Assay Reference Details
Assay Comments by IEDB CuratorT cells from mice immunized with the source antigen proliferated in response to the epitope.
Location of Assay Data in ReferenceFigure 2