Reference | ||
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Reference Type | Literature | IEDB_Reference:1003293 |
Title | Identification of T cell stimulatory epitopes from the 18 kDa protein of Mycobacterium leprae. | |
Authors | T M Doherty; B T Bäckström; S G Love; D R Harding; J D Watson | |
Affiliations | Department of Molecular Medicine, University of Auckland School of Medicine, New Zealand. | |
Journal | Int Immunol | |
Year | 1993 | |
Abstract | We have used different mouse strains to examine in vivo and in vitro responses to the 18 kDa protein of Mycobacterium leprae, which appears to be strongly immunogenic in both mice and humans. B and T cell stimulatory epitopes recognised by different strains of mice have been mapped using overlapping peptides that span the entire 18 kDa protein. Previous work established that immunization of mice with the 18 kDa protein results in specific antibody production to common B cell epitopes and immunization of mice with peptides containing these B cell epitopes resulted in the induction of specific IgG to only a limited subset of epitopes in each strain. Now we report that T cells purified from mice immunized with peptides that stimulate antibody production, proliferate in vitro when rechallenged. The proliferating T cells produce levels of IL-2 and IFN-gamma, that indicate antigen-specific T helper type 1 cells are present in significant numbers. Thus, a comparison of in vivo and in vitro data suggests that T cells bearing the phenotype associated with potentially protective cell-mediated responses can be primed in vivo by epitopes on small peptides. Since T cells from both strains of mice are capable of responding to the immunogenic synthetic peptides in vitro, but give different responses to the same peptides in vivo, factors other than epitope structure appear to influence T cell subset activation. This may have important implications for diseases such as leprosy where a polarized T cell response appears to develop and for the development of synthetic subunit vaccines. | |
Curation Last Updated | 2023-11-21 22:20:51 |
Epitope | ||
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Epitope ID | 13941 | IEDB_epitope:13941 |
Chemical Type | Linear peptide | |
Linear Sequence | EQVLGTSARPAVMPMDAWRE | |
Source Molecule Name | 18 kDa antigen | |
Source Organism | Mycobacterium leprae | |
Starting Position | 16 | |
Ending Position | 35 |
Epitope Reference Details | ||
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Epitope Structure Defines | Epitope containing region/antigenic site | |
Epitope Name | M. leprae 18 kDa protein amino acid residues 16-35 | |
Reference Starting Position | 16 | |
Reference Ending Position | 35 | |
Location of Data in Reference | Figure 3 |
Immunization | ||
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Host Organism | Mus musculus B10.BR |
Host Details | ||
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Age | 6-10 weeks | |
MHC Types present | H2-k class I;H2-k class II |
1st In Vivo Process | ||
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In Vivo Process Type | Administration in vivo |
Administration Details | ||
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Adjuvants | Freund's incomplete (IFA); | VO:0000142 |
Route | subcutaneous |
1st Immunogen | ||
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Epitope Relation | Source Antigen | |
Chemical Type | Protein | |
Molecule Name | 18 kDa antigen | |
Organism | Mycobacterium leprae |
Immunogen Details | ||
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Immunogen Reference Name | 18 kDa antigen |
Immunization Comments | ||
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Immunization Comments | Antigen was dissolved in PBS then emulsified in IFA (50:50 v/v) then given via the hind footpads (25 ul each) and at the base of the tail (50 ul). T cells were prepared 7-14 days after the final immunization. |
T Cell Assay | ||
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Qualitative Measurement | Positive | |
Method/Technique | 3H-thymidine | |
Measurement of | proliferation |
Effector Cells | ||
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Effector Cell Tissue Type | Lymphoid | |
Effector Cell Type | T cell | |
Effector Cell Culture Conditions | Direct Ex Vivo |
Antigen Presenting Cells | ||
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Cell Tissue Type | Bone Marrow | |
Cell Type | Macrophage | |
Cell Culture Conditions | Direct Ex Vivo |
MHC Allele | ||
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MHC Allele Name | H2-k class II | |
MHC Evidence Code | T cell assay -Biological process measured |
Antigen | ||
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Epitope Relation | Epitope | |
Chemical Type | Linear peptide | |
Linear Sequence | EQVLGTSARPAVMPMDAWRE | |
Source Molecule Name | 18 kDa antigen | |
Source Organism | Mycobacterium leprae | |
Starting Position | 16 | |
Ending Position | 35 |
Antigen Details | ||
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Antigen Reference Name | M. leprae 18 kDa protein amino acid residues 16-35 |
Assay Reference Details | ||
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Assay Comments by IEDB Curator | T cells from mice immunized with the source antigen proliferated in response to the epitope. | |
Location of Assay Data in Reference | Figure 2 |