Reference
Reference TypeLiterature
TitleAntimicrobial activity of MHC class I-restricted CD8+ T cells in human tuberculosis.
AuthorsS Cho; V Mehra; S Thoma-Uszynski; S Stenger; N Serbina; R J Mazzaccaro; J L Flynn; P F Barnes; S Southwood; E Celis; B R Bloom; R L Modlin; A Sette
AffiliationsDivision of Dermatology, University of California School of Medicine, Los Angeles, CA 90095, USA.
JournalProc Natl Acad Sci U S A
Year2000
AbstractStudies of mouse models of tuberculosis (TB) infection have indicated a central role for MHC class I-restricted CD8+ T cells in protective immunity. To define antigens and epitopes of Mycobacterium tuberculosis (MTB) proteins that are presented by infected cells to CD8+ T cells, we screened 40 MTB proteins for HLA class I A*0201-binding motifs. Peptides that bound with high affinity to purified HLA molecules were subsequently analyzed for recognition by CD8+ cytotoxic T lymphocytes. We identified three epitopes recognized by CD8+ T cells from patients recovering from TB infection. Those three epitopes were derived from three different antigens: thymidylate synthase (ThyA(30-38)), RNA polymerase beta-subunit (RpoB(127-135)), and a putative phosphate transport system permease protein A-1 (PstA1(75-83)). In addition, CD8+ T cell lines specific for three peptides (ThyA(30-38), PstA1(75-83), and 85B(15-23)) were generated from peripheral blood mononuclear cells of normal HLA-A*0201 donors. These CD8+ T cell lines specifically recognized MTB-infected macrophages, as demonstrated by production of IFN-gamma and lysis of the infected target cells. Finally, CD8+ cytotoxic T lymphocytes reduced the viability of the intracellular MTB, providing evidence that CD8+ T cell recognition of MHC class I-restricted epitopes of these MTB antigens can contribute to effective immunity against the pathogen.
Curation Last Updated2023-12-29 22:42:46
Epitope
Epitope ID34866
Chemical TypeLinear peptide
Linear SequenceLALVLLMLPV
Source Molecule NamePstA-1
Source OrganismMycobacterium tuberculosis
Starting Position164
Ending Position173
Epitope Reference Details
Epitope Structure DefinesExact Epitope
Epitope NamePstA1 268-277
Reference Starting Position268
Reference Ending Position277
Location of Data in ReferenceSupplementary table
In Vivo Processing
Host OrganismHomo sapiens (human)
MHC Ligand Assay
Qualitative MeasurementPositive-Intermediate
Method/Techniquepurified MHC/competitive/radioactivity
Measurement ofdissociation constant KD (~IC50)
Assay Type UnitsnM
Measurement Details
Quantitative measurement333.3
MHC Allele
MHC Allele NameHLA-A*02:01
Assay Reference Details
Location of Assay Data in ReferenceSupplementary tables