Reference | ||
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Reference Type | Literature | IEDB_Reference:1012770 |
Title | Epitopes of the mycobacterial heat shock protein 65 for human T cells comprise different structures. | |
Authors | M E Munk; T M Shinnick; S H Kaufmann | |
Affiliations | Department of Medical Microbiology and Immunology, University of Ulm, Federal Republic of Germany. | |
Journal | Immunobiology | |
Year | 1990 | |
Abstract | T cell recognition of foreign antigens is a result of a ternary complex between T cell receptor, nominal peptide and major histocompatibility complex molecule. It has been proposed that the nominal peptide, which is presented by accessory cells to T cells, has a characteristic structure which can be predicted on the basis of physicochemical criteria. To further study this aspect, we stimulated T cells from normal human blood donors with synthetic peptides (each of approximately 15 amino acids in length) from the heat shock protein 65 of Mycobacterium tuberculosis-M. bovis. We found that while the characterization of certain epitopes follows commonly used predictions, other epitopes cannot be predicted by known methods. | |
Curation Last Updated | 2023-08-18 20:35:14 |
Epitope | ||
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Epitope ID | 78960 | IEDB_epitope:78960 |
Chemical Type | Linear peptide | |
Linear Sequence | EKIGAELVKEVAKK | |
Source Molecule Name | 60 kDa chaperonin 2 | |
Source Organism | Mycobacterium tuberculosis | |
Starting Position | 66 | |
Ending Position | 79 |
Epitope Reference Details | ||
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Epitope Structure Defines | Epitope containing region/antigenic site | |
Epitope Name | Hsp60 residues 67-78 | |
Reference Starting Position | 67 | |
Reference Ending Position | 78 | |
Location of Data in Reference | Table 1 |
Immunization | ||
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Host Organism | Homo sapiens (human) |
Host Details | ||
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MHC Types present | HLA-DR7;HLA-DR53;HLA-DQ2;HLA-DQ3 |
1st In Vivo Process | ||
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In Vivo Process Type | Exposure with existing immune reactivity without evidence for disease |
1st Immunogen | ||
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Epitope Relation | Source Organism | |
Object Type | Organism | |
Organism | Mycobacterium tuberculosis |
Immunization Comments | ||
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Immunization Comments | PBMCs were from 2 healthy PPD positive individuals. |
T Cell Assay | ||
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Qualitative Measurement | Positive | |
Method/Technique | 3H-thymidine | |
Measurement of | proliferation |
Measurement Details | ||
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Number of Subjects Tested | 2 | |
Number of Subjects Responded | 1 | |
Response Frequency (%) | 50 |
Effector Cells | ||
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Effector Cell Tissue Type | Blood | |
Effector Cell Type | PBMC | |
Effector Cell Culture Conditions | Direct Ex Vivo |
Antigen Presenting Cells | ||
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Cell Tissue Type | Blood | |
Cell Type | PBMC | |
Cell Culture Conditions | Direct Ex Vivo |
MHC Allele | ||
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MHC Allele Name | HLA class II | |
MHC Evidence Code | T cell assay -Biological process measured |
Antigen | ||
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Epitope Relation | Epitope | |
Chemical Type | Linear peptide | |
Linear Sequence | EKIGAELVKEVAKK | |
Source Molecule Name | 60 kDa chaperonin 2 | |
Source Organism | Mycobacterium tuberculosis | |
Starting Position | 66 | |
Ending Position | 79 |
Assay Reference Details | ||
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Location of Assay Data in Reference | Table 1 |