Reference
Reference TypeLiterature
TitleEpitopes of the mycobacterial heat shock protein 65 for human T cells comprise different structures.
AuthorsM E Munk; T M Shinnick; S H Kaufmann
AffiliationsDepartment of Medical Microbiology and Immunology, University of Ulm, Federal Republic of Germany.
JournalImmunobiology
Year1990
AbstractT cell recognition of foreign antigens is a result of a ternary complex between T cell receptor, nominal peptide and major histocompatibility complex molecule. It has been proposed that the nominal peptide, which is presented by accessory cells to T cells, has a characteristic structure which can be predicted on the basis of physicochemical criteria. To further study this aspect, we stimulated T cells from normal human blood donors with synthetic peptides (each of approximately 15 amino acids in length) from the heat shock protein 65 of Mycobacterium tuberculosis-M. bovis. We found that while the characterization of certain epitopes follows commonly used predictions, other epitopes cannot be predicted by known methods.
Curation Last Updated2023-08-18 20:35:14
Epitope
Epitope ID78960
Chemical TypeLinear peptide
Linear SequenceEKIGAELVKEVAKK
Source Molecule Name60 kDa chaperonin 2
Source OrganismMycobacterium tuberculosis
Starting Position66
Ending Position79
Epitope Reference Details
Epitope Structure DefinesEpitope containing region/antigenic site
Epitope NameHsp60 residues 67-78
Reference Starting Position67
Reference Ending Position78
Location of Data in ReferenceTable 1
Immunization
Host OrganismHomo sapiens (human)
Host Details
MHC Types presentHLA-DR7;HLA-DR53;HLA-DQ2;HLA-DQ3
1st In Vivo Process
In Vivo Process TypeExposure with existing immune reactivity without evidence for disease
1st Immunogen
Epitope RelationSource Organism
Object TypeOrganism
OrganismMycobacterium tuberculosis
Immunization Comments
Immunization CommentsPBMCs were from 2 healthy PPD positive individuals.
T Cell Assay
Qualitative MeasurementPositive
Method/Technique3H-thymidine
Measurement ofproliferation
Measurement Details
Number of Subjects Tested2
Number of Subjects Responded1
Response Frequency (%)50
Effector Cells
Effector Cell Tissue TypeBlood
Effector Cell TypePBMC
Effector Cell Culture ConditionsDirect Ex Vivo
Antigen Presenting Cells
Cell Tissue TypeBlood
Cell TypePBMC
Cell Culture ConditionsDirect Ex Vivo
MHC Allele
MHC Allele NameHLA class II
MHC Evidence CodeT cell assay -Biological process measured
Antigen
Epitope RelationEpitope
Chemical TypeLinear peptide
Linear SequenceEKIGAELVKEVAKK
Source Molecule Name60 kDa chaperonin 2
Source OrganismMycobacterium tuberculosis
Starting Position66
Ending Position79
Assay Reference Details
Location of Assay Data in ReferenceTable 1