| Reference | ||
|---|---|---|
| Reference Type | Literature | IEDB_Reference:1125 |
| Title | Human T-cell responses to the RD1-encoded protein TB27.4 (Rv3878) from Mycobacterium tuberculosis. | |
| Authors | Else Marie Agger; Inger Brock; Limei Meng Okkels; Sandra M Arend; Claus S Aagaard; Karin N Weldingh; Peter Andersen | |
| Affiliations | Department of Infectious Disease Immunology, Statens Serum Institut, Copenhagen, Denmark. eag@ssi.dk. | |
| Journal | Immunology | |
| Year | 2003 | |
| Abstract | In recent years, there has been considerable focus on the discovery and characterization of proteins derived from Mycobacterium tuberculosis leading to the identification of a number of candidate antigens for use in vaccine development or for diagnostic purposes. Previous experiments have demonstrated an important immunological role for proteins encoded by the RD1 region, which is absent from all strains of bacillus Calmette-Guérin (BCG) but present in the genomes of virulent M. bovis and M. tuberculosis. Herein, we have studied human T-cell responses to the antigen encoded by the putative open reading frame (rv3878) of the RD1 region. Immunoblot analysis revealed that rv3878 was expressed and the native protein was designated TB27.4. Immunological evaluations demonstrate that TB27.4 elicits a prominent immune response in human tuberculosis patients with a dominant region in the C-terminal part of the molecule. In contrast, very limited responses were seen in M. bovis BCG-vaccinated donors. This study therefore emphasizes the diagnostic potential of proteins encoded by the RD1 region. | |
| Curation Last Updated | 2023-08-18 20:02:45 | |
| Epitope | ||
|---|---|---|
| Epitope ID | 1452 | IEDB_epitope:1452 |
| Chemical Type | Linear peptide | |
| Linear Sequence | AGARPAASPLAAPVDPSTPA | |
| Source Molecule Name | hypothetical protein Rv3878 - Mycobacterium tuberculosis (strain H37RV) | |
| Source Organism | Mycobacterium tuberculosis H37Rv (Mycobacterium tuberculosis str. H37Rv) | |
| Starting Position | 254 | |
| Ending Position | 273 | |
| Epitope Reference Details | ||
|---|---|---|
| Epitope Structure Defines | Epitope containing region/antigenic site | |
| Epitope Name | p22 | |
| Reference Starting Position | 254 | |
| Reference Ending Position | 273 | |
| Location of Data in Reference | Table 1 | |
| Immunization | ||
|---|---|---|
| Host Organism | Homo sapiens (human) | |
| 1st In Vivo Process | ||
|---|---|---|
| In Vivo Process Type | Occurrence of infectious disease | |
| Disease State | tuberculosis | |
| Disease Stage | Unknown; | |
| 1st Immunogen | ||
|---|---|---|
| Epitope Relation | Source Organism | |
| Object Type | Organism | |
| Organism | Mycobacterium tuberculosis H37Rv (Mycobacterium tuberculosis str. H37Rv) | |
| Immunogen Details | ||
|---|---|---|
| Immunogen Reference Name | Mycobacterium tuberculosis H37Rv | |
| T Cell Assay | ||
|---|---|---|
| Qualitative Measurement | Positive | |
| Method/Technique | ELISA | |
| Measurement of | IFNg release | |
| Effector Cells | ||
|---|---|---|
| Effector Cell Tissue Type | Blood | |
| Effector Cell Type | PBMC | |
| Effector Cell Culture Conditions | Direct Ex Vivo | |
| Antigen Presenting Cells | ||
|---|---|---|
| Cell Tissue Type | Blood | |
| Cell Type | PBMC | |
| Cell Culture Conditions | Direct Ex Vivo | |
| Antigen | ||
|---|---|---|
| Epitope Relation | Epitope | |
| Chemical Type | Linear peptide | |
| Linear Sequence | AGARPAASPLAAPVDPSTPA | |
| Source Molecule Name | hypothetical protein Rv3878 - Mycobacterium tuberculosis (strain H37RV) | |
| Source Organism | Mycobacterium tuberculosis H37Rv (Mycobacterium tuberculosis str. H37Rv) | |
| Starting Position | 254 | |
| Ending Position | 273 | |
| Antigen Details | ||
|---|---|---|
| Antigen Reference Name | p22 | |
| Assay Reference Details | ||
|---|---|---|
| Assay Comments by IEDB Curator | BCG-vaccinated Caucasian Danes with no known exposure or history of TB showed very limited IFN-γ response. No reactivity to the peptide was seen in unvaccinated, PPD-negative Caucasian Danes. | |
| Location of Assay Data in Reference | Figure 3 | |