Reference
Reference TypeLiterature
TitlePeptide-based antibody detection for tuberculosis diagnosis.
AuthorsGuomiao Shen; Digambar Behera; Manpreet Bhalla; Arthur Nadas; Suman Laal
AffiliationsDepartment of Pathology, New York University School of Medicine, New York, NY 10010, USA.
JournalClin Vaccine Immunol
Year2009
AbstractTuberculosis (TB) is a major cause of morbidity and mortality, especially in developing countries. Despite significant limitations, microscopy remains the cornerstone of the global TB control strategy. As the TB epidemic escalates, new diagnostic methods that are accurate and also economical and simple to manufacture and deploy are urgently needed. Although several promising antigens have been identified and evaluated in recent years, the reproducible production of high-quality recombinant mycobacterial proteins with minimal batch-to-batch variation is difficult, laborious, and expensive. To determine the feasibility of devising a synthetic peptide-based diagnostic test for TB, we have delineated the immunodominant epitopes of three candidate antigens, Ag85B, BfrB, and TrxC, that were previously identified to be immunogenic in TB patients. The results demonstrate that combinations of carefully selected synthetic peptides derived from highly immunogenic proteins can be the basis for devising an immunodiagnostic test for TB.
Curation Last Updated2023-08-18 20:38:39
Epitope
Epitope ID17838
Chemical TypeLinear peptide
Linear SequenceFSRPGLPVEYLQVPSPSMGR
Source Molecule NameAntigen 85-B
Source OrganismMycobacterium tuberculosis
Starting Position41
Ending Position60
Epitope Reference Details
Epitope Structure DefinesEpitope containing region/antigenic site
Epitope NameAg85B A5
CommentsThe epitope sequene was cited in Anderson et al. (2001) J. Mol. Biol. 307 (2), 671-681 [PMID: 12218036].
Location of Data in ReferenceCited reference
Immunization
Host OrganismHomo sapiens (human)
Host Details
Host GeolocationIndia
1st In Vivo Process
In Vivo Process TypeOccurrence of infectious disease
Disease Statetuberculosis
Disease StageUnknown;
1st Immunogen
Epitope RelationSource Organism
Object TypeOrganism
OrganismMycobacterium tuberculosis
Immunization Comments
Immunization CommentsSerum from patients who were diagnosed with TB on the basis of the fact that their sputum smears were positive for acid-fast bacilli were used in this study.
B Cell Assay
Qualitative MeasurementPositive-Low
Method/TechniqueELISA
Measurement ofqualitative binding
Measurement Details
Number of Subjects Tested60
Assayed Antibody
Assayed Antibody Source MaterialSerum
Assayed Antibody Immunoglobulin DomainEntire Antibody
Assayed Antibody Purification StatusPolyclonal
Antigen
Epitope RelationEpitope
Chemical TypeLinear peptide
Linear SequenceFSRPGLPVEYLQVPSPSMGR
Source Molecule NameAntigen 85-B
Source OrganismMycobacterium tuberculosis
Starting Position41
Ending Position60
Assay Reference Details
Location of Assay Data in ReferenceFigure 1