Reference | ||
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Reference Type | Literature | IEDB_Reference:1013688 |
Title | Peptide-based antibody detection for tuberculosis diagnosis. | |
Authors | Guomiao Shen; Digambar Behera; Manpreet Bhalla; Arthur Nadas; Suman Laal | |
Affiliations | Department of Pathology, New York University School of Medicine, New York, NY 10010, USA. | |
Journal | Clin Vaccine Immunol | |
Year | 2009 | |
Abstract | Tuberculosis (TB) is a major cause of morbidity and mortality, especially in developing countries. Despite significant limitations, microscopy remains the cornerstone of the global TB control strategy. As the TB epidemic escalates, new diagnostic methods that are accurate and also economical and simple to manufacture and deploy are urgently needed. Although several promising antigens have been identified and evaluated in recent years, the reproducible production of high-quality recombinant mycobacterial proteins with minimal batch-to-batch variation is difficult, laborious, and expensive. To determine the feasibility of devising a synthetic peptide-based diagnostic test for TB, we have delineated the immunodominant epitopes of three candidate antigens, Ag85B, BfrB, and TrxC, that were previously identified to be immunogenic in TB patients. The results demonstrate that combinations of carefully selected synthetic peptides derived from highly immunogenic proteins can be the basis for devising an immunodiagnostic test for TB. | |
Curation Last Updated | 2023-08-18 20:38:39 |
Epitope | ||
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Epitope ID | 17838 | IEDB_epitope:17838 |
Chemical Type | Linear peptide | |
Linear Sequence | FSRPGLPVEYLQVPSPSMGR | |
Source Molecule Name | Antigen 85-B | |
Source Organism | Mycobacterium tuberculosis | |
Starting Position | 41 | |
Ending Position | 60 |
Epitope Reference Details | ||
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Epitope Structure Defines | Epitope containing region/antigenic site | |
Epitope Name | Ag85B A5 | |
Comments | The epitope sequene was cited in Anderson et al. (2001) J. Mol. Biol. 307 (2), 671-681 [PMID: 12218036]. | |
Location of Data in Reference | Cited reference |
Immunization | ||
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Host Organism | Homo sapiens (human) |
Host Details | ||
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Host Geolocation | India |
1st In Vivo Process | ||
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In Vivo Process Type | Occurrence of infectious disease | |
Disease State | tuberculosis | |
Disease Stage | Unknown; |
1st Immunogen | ||
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Epitope Relation | Source Organism | |
Object Type | Organism | |
Organism | Mycobacterium tuberculosis |
Immunization Comments | ||
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Immunization Comments | Serum from patients who were diagnosed with TB on the basis of the fact that their sputum smears were positive for acid-fast bacilli were used in this study. |
B Cell Assay | ||
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Qualitative Measurement | Positive-Low | |
Method/Technique | ELISA | |
Measurement of | qualitative binding |
Measurement Details | ||
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Number of Subjects Tested | 60 |
Assayed Antibody | ||
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Assayed Antibody Source Material | Serum | |
Assayed Antibody Immunoglobulin Domain | Entire Antibody | |
Assayed Antibody Purification Status | Polyclonal |
Antigen | ||
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Epitope Relation | Epitope | |
Chemical Type | Linear peptide | |
Linear Sequence | FSRPGLPVEYLQVPSPSMGR | |
Source Molecule Name | Antigen 85-B | |
Source Organism | Mycobacterium tuberculosis | |
Starting Position | 41 | |
Ending Position | 60 |
Assay Reference Details | ||
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Location of Assay Data in Reference | Figure 1 |