Reference
Reference TypeLiterature
TitleStructural features of autoreactive TCR that determine the degree of degeneracy in peptide recognition.
AuthorsS Hausmann; M Martin; L Gauthier; K W Wucherpfennig
AffiliationsDepartment of Cancer Immunology and AIDS, Dana-Farber Cancer Institute, Boston, MA 02115, USA.
JournalJ Immunol
Year1999
AbstractStructural aspects of human TCRs that allow the activation of autoreactive T cells by diverse microbial peptides were examined using two human myelin basic protein (MBP)-specific T cell clones. The TCR sequences of these clones differed only in the N region of TCR-alpha and -beta since the clones had the same Valpha-Jalpha and Vbeta-Jbeta rearrangements. The two clones had a similar fine specificity for the MBP peptide, except for the P5 position of the peptide (lysine). In the crystal structure of the HLA-DR2/MBP peptide complex, P5 lysine is a prominent, solvent-exposed residue in the center of the DR2/MBP peptide surface. Five microbial peptides with conservative or nonconservative changes at the P5 position (lysine to arginine, serine, or proline) activated one of these clones. In contrast, the other clone was activated only by three of these peptides which had a conservative lysine to arginine change at P5. The degree of specificity/degeneracy in recognition of the P5 side chain was the key difference between these TCRs since the Escherichia coli/Haemophilus influenzae peptide stimulated both clones when the P5 position was substituted from serine to arginine. These results demonstrate that the complementarity-determining region 3 loops contribute to the degree of degeneracy in peptide recognition by human MBP-specific TCRs.
Curation Last Updated2023-08-18 20:32:31
Epitope
Epitope ID115927
Chemical TypeLinear peptide
Linear SequenceQRCRVHFMRNLYTAV
Source Molecule Namehypothetical protein
Source OrganismMycobacterium tuberculosis
Starting Position229
Ending Position243
Epitope Reference Details
Epitope Structure DefinesExact Epitope
Epitope NameS35
Location of Data in ReferenceTable IV
In Vivo Processing
Host OrganismHomo sapiens (human)
MHC Ligand Assay
Qualitative MeasurementPositive
Method/Techniquepurified MHC/competitive/fluorescence
Measurement ofqualitative binding
MHC Allele
MHC Allele NameHLA-DRB1*15:01
Assay Reference Details
Location of Assay Data in ReferenceFigure 3