Reference | ||
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Reference Type | Submission | IEDB_Reference:1019521 |
Title | Large scale analysis of peptide-HLA class I interactions | |
Authors | Mikkel Harndahl; Kasper Lamberth Ph.D.; Gustav Roder; Sune Justesen; Michael Madsen; Morten Nielsen; Claus Lundegaard; Mette Voldby Larsen; Sheila Tang; Soren Brunak; Ole Lund; Soren Buus | |
Affiliations | Div of Experimental Immunology, Dep Med Microbiol Immunol, University of Copenhagen, Cntr Biol Seq Anal, Technical University of Denmark. | |
Submitter | Soren Buus | IEDB_Submission:1000464 |
Year | 2010 | |
Abstract | In general, this data has been generated using a quantitative biochemical peptide-HLA interaction assay (see PMID: 12135423). The HLA class I molecules have been selected to cover the known HLA supertypes (using PMID: 14963618). Predictions of peptide binding to HLA class I has been performed using various bioinformatics approaches including artificial neural networks (see PMID: 14617044 and PMID: 12717023 peptides and using a combined algorithm integrating MHC class I binding, TAP transport efficiency, and proteasomal cleavage predictions (PMID: 15997466). In many cases, the most informative peptides have been selected using a Query-by-Committeee procedure (using PMID: 14617044 and PMID: 14629874). | |
Curation Last Updated | 2023-08-18 20:52:00 |
Epitope | ||
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Epitope ID | 126076 | IEDB_epitope:126076 |
Chemical Type | Linear peptide | |
Linear Sequence | FVYSVSFHK | |
Source Molecule Name | hypothetical protein | |
Source Organism | Mycobacterium tuberculosis C (Mycobacterium tuberculosis str. C) | |
Starting Position | 55 | |
Ending Position | 63 |
Epitope Reference Details | ||
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Epitope Structure Defines | Exact Epitope | |
Epitope Name | 99108 |
In Vivo Processing | ||
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Host Organism | Homo sapiens (human) |
MHC Ligand Assay | ||
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Qualitative Measurement | Positive-Low | |
Method/Technique | purified MHC/competitive/fluorescence | |
Measurement of | dissociation constant KD | |
Assay Type Units | nM |
Measurement Details | ||
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Measurement Inequality | > | |
Quantitative measurement | 5000 |
MHC Allele | ||
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MHC Allele Name | HLA-B*83:01 |
Assay Reference Details | ||
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Assay Comments by IEDB Curator | In general, this data has been generated using a quantitative biochemical peptide-HLA interaction assay (see PMID: 12135423). The HLA class I molecules have been selected to cover the known HLA supertypes (using PMID: 14963618). Predictions of peptide binding to HLA class I has been performed using various bioinformatics approaches including artificial neural networks (see PMID: 14617044 �and PMID: 12717023 peptides and using a combined algorithm integrating MHC class I binding, TAP transport efficiency, and proteasomal cleavage predictions (PMID: 15997466). In many cases, the most informative peptides have been selected using a Query-by-Committeee procedure (using PMID: 14617044 and PMID: 14629874). |