Reference
Reference TypeSubmission
TitleLarge scale analysis of peptide-HLA class I interactions
AuthorsMikkel Harndahl; Kasper Lamberth Ph.D.; Gustav Roder; Sune Justesen; Michael Madsen; Morten Nielsen; Claus Lundegaard; Mette Voldby Larsen; Sheila Tang; Soren Brunak; Ole Lund; Soren Buus
AffiliationsDiv of Experimental Immunology, Dep Med Microbiol Immunol, University of Copenhagen, Cntr Biol Seq Anal, Technical University of Denmark.
SubmitterSoren Buus
Year2010
AbstractIn general, this data has been generated using a quantitative biochemical peptide-HLA interaction assay (see PMID: 12135423). The HLA class I molecules have been selected to cover the known HLA supertypes (using PMID: 14963618). Predictions of peptide binding to HLA class I has been performed using various bioinformatics approaches including artificial neural networks (see PMID: 14617044 and PMID: 12717023 peptides and using a combined algorithm integrating MHC class I binding, TAP transport efficiency, and proteasomal cleavage predictions (PMID: 15997466). In many cases, the most informative peptides have been selected using a Query-by-Committeee procedure (using PMID: 14617044 and PMID: 14629874).
Curation Last Updated2023-08-18 20:52:00
Epitope
Epitope ID126076
Chemical TypeLinear peptide
Linear SequenceFVYSVSFHK
Source Molecule Namehypothetical protein
Source OrganismMycobacterium tuberculosis C (Mycobacterium tuberculosis str. C)
Starting Position55
Ending Position63
Epitope Reference Details
Epitope Structure DefinesExact Epitope
Epitope Name99108
In Vivo Processing
Host OrganismHomo sapiens (human)
MHC Ligand Assay
Qualitative MeasurementPositive-Low
Method/Techniquepurified MHC/competitive/fluorescence
Measurement ofdissociation constant KD
Assay Type UnitsnM
Measurement Details
Measurement Inequality>
Quantitative measurement5000
MHC Allele
MHC Allele NameHLA-B*83:01
Assay Reference Details
Assay Comments by IEDB CuratorIn general, this data has been generated using a quantitative biochemical peptide-HLA interaction assay (see PMID: 12135423). The HLA class I molecules have been selected to cover the known HLA supertypes (using PMID: 14963618). Predictions of peptide binding to HLA class I has been performed using various bioinformatics approaches including artificial neural networks (see PMID: 14617044 �and PMID: 12717023 peptides and using a combined algorithm integrating MHC class I binding, TAP transport efficiency, and proteasomal cleavage predictions (PMID: 15997466). In many cases, the most informative peptides have been selected using a Query-by-Committeee procedure (using PMID: 14617044 and PMID: 14629874).