Reference
Reference TypeLiterature
TitleProtective immunity against Mycobacterium tuberculosis induced by dendritic cells pulsed with both CD8(+)- and CD4(+)-T-cell epitopes from antigen 85A.
AuthorsHelen McShane; Shahriar Behboudi; Nilu Goonetilleke; Roger Brookes; Adrian V S Hill
AffiliationsNuffield Department of Medicine, University of Oxford, John Radcliffe Hospital, Oxford OX3 9DU, United Kingdom. helen.mcshane@ndm.ox.ac.uk.
JournalInfect Immun
Year2002
AbstractImmunization with DNA followed by modified vaccinia virus Ankara strain, both expressing the antigen 85A, induced both CD4(+)- and CD8(+)-T-cell responses in BALB/c mice. Following challenge with Mycobacterium tuberculosis, this prime-boost regimen produced protection equivalent to that conferred by Mycobacterium bovis BCG. Following immunization with dendritic cells pulsed with an antigen 85A CD4(+)- or CD8(+)-restricted epitope, alone or in combination, copresentation of both epitopes on the same dendritic cell was required for protection, demonstrating that induced CD8(+) T cells can play a protective role against tuberculosis.
Curation Last Updated2023-08-18 20:01:54
Epitope
Epitope ID63707
Chemical TypeLinear peptide
Linear SequenceTFLTSELPGWLQANRHVKPT
Source Molecule NameAntigen 85-A precursor
Source OrganismMycobacterium tuberculosis H37Rv (Mycobacterium tuberculosis str. H37Rv)
Starting Position142
Ending Position161
Epitope Reference Details
Epitope Structure DefinesEpitope containing region/antigenic site
Epitope Namep15
Location of Data in ReferenceTable 1
Immunization
Host OrganismMus musculus BALB/c
Host Details
SexF
Age4-6 weeks old
1st In Vivo Process
In Vivo Process TypeAdministration in vivo
Administration Details
Routeintramuscular
Dose ScheduleBCG Glaxo was injected intradermally
1st Immunogen
Epitope RelationSource Antigen
Chemical TypeProtein
Molecule NameAntigen 85-A precursor
OrganismMycobacterium tuberculosis H37Rv (Mycobacterium tuberculosis str. H37Rv)
Immunogen Details
Immunogen Reference NameAntigen 85A
In Vitro Administration
In Vitro Process TypeRestimulation in vitro
Immunization Comments
Immunization CommentsImmunogen carrier: Plasmid. In addition to the plasmid vector expressing the 85A antigen, recombinant vaccinia virus Ankara strain expressing the 85A antigen was injected intravenously as a second immunogen in a prime-boost regimen.
T Cell Assay
Qualitative MeasurementPositive
Method/TechniqueELISPOT
Measurement ofIFNg release
Measurement Details
Quantitative measurement250
Effector Cells
Effector Cell Tissue TypeSpleen
Effector Cell TypeT cell CD4+
Effector Cell Culture ConditionsShort Term Restimulated
Antigen Presenting Cells
Cell Tissue TypeSpleen
Cell TypeSplenocyte
Cell Culture ConditionsDirect Ex Vivo
MHC Allele
MHC Allele NameH2-d class II
MHC Evidence CodeNot determined
Antigen
Epitope RelationEpitope
Chemical TypeLinear peptide
Linear SequenceTFLTSELPGWLQANRHVKPT
Source Molecule NameAntigen 85-A precursor
Source OrganismMycobacterium tuberculosis H37Rv (Mycobacterium tuberculosis str. H37Rv)
Starting Position142
Ending Position161
Antigen Details
Antigen Reference Namep15
Assay Reference Details
Assay Comments by IEDB CuratorThe response remained strong when splenocytes were depleted of CD8+ cells, but was eliminated when the experiment was performed with CD4+ depleted splenocytes, signifying a CD4+-restricted epitope. Splenocytes were depleted of CD4+ cells.
Location of Assay Data in ReferenceTable 1