Reference | ||
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Reference Type | Literature | IEDB_Reference:695 |
Title | Protective immunity against Mycobacterium tuberculosis induced by dendritic cells pulsed with both CD8(+)- and CD4(+)-T-cell epitopes from antigen 85A. | |
Authors | Helen McShane; Shahriar Behboudi; Nilu Goonetilleke; Roger Brookes; Adrian V S Hill | |
Affiliations | Nuffield Department of Medicine, University of Oxford, John Radcliffe Hospital, Oxford OX3 9DU, United Kingdom. helen.mcshane@ndm.ox.ac.uk. | |
Journal | Infect Immun | |
Year | 2002 | |
Abstract | Immunization with DNA followed by modified vaccinia virus Ankara strain, both expressing the antigen 85A, induced both CD4(+)- and CD8(+)-T-cell responses in BALB/c mice. Following challenge with Mycobacterium tuberculosis, this prime-boost regimen produced protection equivalent to that conferred by Mycobacterium bovis BCG. Following immunization with dendritic cells pulsed with an antigen 85A CD4(+)- or CD8(+)-restricted epitope, alone or in combination, copresentation of both epitopes on the same dendritic cell was required for protection, demonstrating that induced CD8(+) T cells can play a protective role against tuberculosis. | |
Curation Last Updated | 2023-08-18 20:01:54 |
Epitope | ||
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Epitope ID | 63707 | IEDB_epitope:63707 |
Chemical Type | Linear peptide | |
Linear Sequence | TFLTSELPGWLQANRHVKPT | |
Source Molecule Name | Antigen 85-A precursor | |
Source Organism | Mycobacterium tuberculosis H37Rv (Mycobacterium tuberculosis str. H37Rv) | |
Starting Position | 142 | |
Ending Position | 161 |
Epitope Reference Details | ||
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Epitope Structure Defines | Epitope containing region/antigenic site | |
Epitope Name | p15 | |
Location of Data in Reference | Table 1 |
Immunization | ||
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Host Organism | Mus musculus BALB/c |
Host Details | ||
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Sex | F | |
Age | 4-6 weeks old |
1st In Vivo Process | ||
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In Vivo Process Type | Administration in vivo |
Administration Details | ||
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Route | intramuscular | |
Dose Schedule | BCG Glaxo was injected intradermally |
1st Immunogen | ||
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Epitope Relation | Source Antigen | |
Chemical Type | Protein | |
Molecule Name | Antigen 85-A precursor | |
Organism | Mycobacterium tuberculosis H37Rv (Mycobacterium tuberculosis str. H37Rv) |
Immunogen Details | ||
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Immunogen Reference Name | Antigen 85A |
In Vitro Administration | ||
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In Vitro Process Type | Restimulation in vitro |
Immunization Comments | ||
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Immunization Comments | Immunogen carrier: Plasmid. In addition to the plasmid vector expressing the 85A antigen, recombinant vaccinia virus Ankara strain expressing the 85A antigen was injected intravenously as a second immunogen in a prime-boost regimen. |
T Cell Assay | ||
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Qualitative Measurement | Positive | |
Method/Technique | ELISPOT | |
Measurement of | IFNg release |
Measurement Details | ||
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Quantitative measurement | 250 |
Effector Cells | ||
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Effector Cell Tissue Type | Spleen | |
Effector Cell Type | T cell CD4+ | |
Effector Cell Culture Conditions | Short Term Restimulated |
Antigen Presenting Cells | ||
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Cell Tissue Type | Spleen | |
Cell Type | Splenocyte | |
Cell Culture Conditions | Direct Ex Vivo |
MHC Allele | ||
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MHC Allele Name | H2-d class II | |
MHC Evidence Code | Not determined |
Antigen | ||
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Epitope Relation | Epitope | |
Chemical Type | Linear peptide | |
Linear Sequence | TFLTSELPGWLQANRHVKPT | |
Source Molecule Name | Antigen 85-A precursor | |
Source Organism | Mycobacterium tuberculosis H37Rv (Mycobacterium tuberculosis str. H37Rv) | |
Starting Position | 142 | |
Ending Position | 161 |
Antigen Details | ||
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Antigen Reference Name | p15 |
Assay Reference Details | ||
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Assay Comments by IEDB Curator | The response remained strong when splenocytes were depleted of CD8+ cells, but was eliminated when the experiment was performed with CD4+ depleted splenocytes, signifying a CD4+-restricted epitope. Splenocytes were depleted of CD4+ cells. | |
Location of Assay Data in Reference | Table 1 |