Reference
Reference TypeLiterature
TitleHuman T cell responses to peptides of the Mycobacterium leprae 45-kD serine-rich antigen.
AuthorsS Brahmbhatt; R Hussain; S Zafar; G Dawood; T H M Ottenhoff; J W Drijfhout; G Bothamley; S Smith; F V Lopez; H M Dockrell
AffiliationsImmunology Unit, Department of Infectious and Tropical Diseases, London School of Hygiene and Tropical Medicine, London, UK.
JournalClin Exp Immunol
Year2002
AbstractIn order to identify T cell epitopes within the Mycobacterium leprae 45-kD serine-rich antigen, we analysed responses to overlapping 17-mer peptides encompassing the whole antigen in non-exposed UK controls, Pakistani leprosy patients and tuberculosis patients in both the United Kingdom and Pakistan. This antigen has been described as M. leprae-specific, although it has a hypothetical homologue in M. tuberculosis. Human peripheral blood mononuclear cells were stimulated with peptide for 5 days and IFN-gamma measured in supernatants by ELISA. Some peptides were recognized more frequently by T cells from tuberculoid leprosy patients than those from UK controls, suggesting that such T cell epitopes might have diagnostic potential, while other peptides induced greater responses among UK control subjects. Short-term cell lines confirmed that these assays detected specific T cell recognition of these peptides. However, many tuberculosis patients also recognized these potentially specific peptides suggesting that there could be a true homologue present in M. tuberculosis.
Curation Last Updated2023-11-21 22:16:03
Epitope
Epitope ID36795
Chemical TypeLinear peptide
Linear SequenceLKAINWFGQVSTTVAAL
Source Molecule NameSerine-rich antigen
Source OrganismMycobacterium leprae
Starting Position121
Ending Position137
Epitope Reference Details
Epitope Structure DefinesEpitope containing region/antigenic site
Epitope Namep21
Location of Data in ReferenceTable 2
Immunization
Host OrganismHomo sapiens (human)
Host Details
Host GeolocationPakistan
1st In Vivo Process
In Vivo Process TypeOccurrence of infectious disease
Disease Stateleprosy
Disease StageChronic;OGMS:0000064
1st Immunogen
Epitope RelationSource Organism
Object TypeOrganism
OrganismMycobacterium leprae
Immunogen Details
Immunogen Reference NameM. Leprae
Immunization Comments
Immunization CommentsPatients were diagnosed and classified by standard clinical and bacteriological parameters and all had BCG scars. All the patients were in the early stages of multi drug therapy (less than 4 weeks' treatment) and none had a previous history of tuberculosis or other diseases.
T Cell Assay
Qualitative MeasurementPositive
Method/TechniqueELISA
Measurement ofIFNg release
Measurement Details
Number of Subjects Tested15
Effector Cells
Effector Cell Tissue TypeBlood
Effector Cell TypePBMC
Effector Cell Culture ConditionsDirect Ex Vivo
Antigen Presenting Cells
Cell Tissue TypeBlood
Cell TypePBMC
Cell Culture ConditionsDirect Ex Vivo
Antigen
Epitope RelationEpitope
Chemical TypeLinear peptide
Linear SequenceLKAINWFGQVSTTVAAL
Source Molecule NameSerine-rich antigen
Source OrganismMycobacterium leprae
Starting Position121
Ending Position137
Antigen Details
Antigen Reference Namep21
Assay Reference Details
Assay Comments by IEDB CuratorPBMC from 27% of TT/BT leprosy patients tested (n=15) had IFNg production after stimulation by the epitope versus 35% of healthy controls.
Location of Assay Data in ReferenceTable 1