Reference | ||
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Reference Type | Literature | IEDB_Reference:787 |
Title | Human T cell responses to peptides of the Mycobacterium leprae 45-kD serine-rich antigen. | |
Authors | S Brahmbhatt; R Hussain; S Zafar; G Dawood; T H M Ottenhoff; J W Drijfhout; G Bothamley; S Smith; F V Lopez; H M Dockrell | |
Affiliations | Immunology Unit, Department of Infectious and Tropical Diseases, London School of Hygiene and Tropical Medicine, London, UK. | |
Journal | Clin Exp Immunol | |
Year | 2002 | |
Abstract | In order to identify T cell epitopes within the Mycobacterium leprae 45-kD serine-rich antigen, we analysed responses to overlapping 17-mer peptides encompassing the whole antigen in non-exposed UK controls, Pakistani leprosy patients and tuberculosis patients in both the United Kingdom and Pakistan. This antigen has been described as M. leprae-specific, although it has a hypothetical homologue in M. tuberculosis. Human peripheral blood mononuclear cells were stimulated with peptide for 5 days and IFN-gamma measured in supernatants by ELISA. Some peptides were recognized more frequently by T cells from tuberculoid leprosy patients than those from UK controls, suggesting that such T cell epitopes might have diagnostic potential, while other peptides induced greater responses among UK control subjects. Short-term cell lines confirmed that these assays detected specific T cell recognition of these peptides. However, many tuberculosis patients also recognized these potentially specific peptides suggesting that there could be a true homologue present in M. tuberculosis. | |
Curation Last Updated | 2023-11-21 22:16:03 |
Epitope | ||
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Epitope ID | 36795 | IEDB_epitope:36795 |
Chemical Type | Linear peptide | |
Linear Sequence | LKAINWFGQVSTTVAAL | |
Source Molecule Name | Serine-rich antigen | |
Source Organism | Mycobacterium leprae | |
Starting Position | 121 | |
Ending Position | 137 |
Epitope Reference Details | ||
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Epitope Structure Defines | Epitope containing region/antigenic site | |
Epitope Name | p21 | |
Location of Data in Reference | Table 2 |
Immunization | ||
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Host Organism | Homo sapiens (human) |
Host Details | ||
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Host Geolocation | Pakistan |
1st In Vivo Process | ||
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In Vivo Process Type | Occurrence of infectious disease | |
Disease State | leprosy | |
Disease Stage | Chronic; | OGMS:0000064 |
1st Immunogen | ||
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Epitope Relation | Source Organism | |
Object Type | Organism | |
Organism | Mycobacterium leprae |
Immunogen Details | ||
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Immunogen Reference Name | M. Leprae |
Immunization Comments | ||
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Immunization Comments | Patients were diagnosed and classified by standard clinical and bacteriological parameters and all had BCG scars. All the patients were in the early stages of multi drug therapy (less than 4 weeks' treatment) and none had a previous history of tuberculosis or other diseases. |
T Cell Assay | ||
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Qualitative Measurement | Positive | |
Method/Technique | ELISA | |
Measurement of | IFNg release |
Measurement Details | ||
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Number of Subjects Tested | 15 |
Effector Cells | ||
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Effector Cell Tissue Type | Blood | |
Effector Cell Type | PBMC | |
Effector Cell Culture Conditions | Direct Ex Vivo |
Antigen Presenting Cells | ||
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Cell Tissue Type | Blood | |
Cell Type | PBMC | |
Cell Culture Conditions | Direct Ex Vivo |
Antigen | ||
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Epitope Relation | Epitope | |
Chemical Type | Linear peptide | |
Linear Sequence | LKAINWFGQVSTTVAAL | |
Source Molecule Name | Serine-rich antigen | |
Source Organism | Mycobacterium leprae | |
Starting Position | 121 | |
Ending Position | 137 |
Antigen Details | ||
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Antigen Reference Name | p21 |
Assay Reference Details | ||
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Assay Comments by IEDB Curator | PBMC from 27% of TT/BT leprosy patients tested (n=15) had IFNg production after stimulation by the epitope versus 35% of healthy controls. | |
Location of Assay Data in Reference | Table 1 |