| Reference | ||
|---|---|---|
| Reference Type | Submission | IEDB_Reference:1027503 |
| Title | Identification of CD8+ T Cell Epitopes in DENV using samples from a Nicaragua cohort | |
| Authors | Daniela Weiskopf Ph.D.; Ruklanthi de Alwis Ph.D.; Sinu Paul Ph.D.; Michael A. Angelo B.Sc; Ravi V. Kolla Ph.D.; John Sidney Ph.D.; Alessandro Sette Ph.D.; Bjoern M. Peters Ph.D. | |
| Affiliations | La Jolla Institute for Allergy and Immunology | |
| Submitter | Daniela Weiskopf | IEDB_Submission:1000582 |
| Year | 2014 | |
| Abstract | Dengue virus (DENV) has been endemic in Nicaragua since 1985, and all four DENV serotypes are found in circulation, generally with one serotype dominating each season. In Managua, the capital of Nicaragua, by age 14, 90% or more of children have been exposed to DENV, suggesting that the vast majority of adults have experienced multiple DENV infections based on serological testing. We utilized a previously described epitope identification approach based on testing predicted epitopes for a panel of the most common HLA-A and B alleles. HLA-matched peptides were utilized to screen peripheral blood mononuclear cells (PBMCs) from individual blood bank donors, repeatedly infected by DENV. Ex vivo IFN-γ responses were measured directly from a total of 130 donors using ELISPOT assays. Reactivity was detected ex vivo for over 30% of the donors. Responses were broad, with an average of 7 epitopes per donor being recognized, and associated with an average magnitude per donor of 212 spot-forming cells (SFC)/106 PBMCs. A total of 520 different responses were detected and mapped back to 314 different individual epitopes. This approach allowed defining responses for the vast majority of alleles expressed in the general Nicaraguan population, and thus characterizing immunodominance at the level of specific HLA loci and allelic variants. | |
| Curation Last Updated | 2023-08-18 21:16:37 | |
| Related Information | |
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| Epitopes | |
| Bcell Assays | 0 |
| Tcell Assays | |
| MHC Ligand Assays | 0 |