Reference
Reference TypeLiterature
TitleGlobal proteogenomic analysis of human MHC class I-associated peptides derived from non-canonical reading frames.
AuthorsCéline M Laumont; Tariq Daouda; Jean-Philippe Laverdure; Éric Bonneil; Olivier Caron-Lizotte; Marie-Pierre Hardy; Diana P Granados; Chantal Durette; Sébastien Lemieux; Pierre Thibault; Claude Perreault
AffiliationsInstitute for Research in Immunology and Cancer, Université de Montréal, PO Box 6128 Station Centre-Ville, Montreal, Quebec, Canada H3C 3J7; Department of Medicine, Faculty of Medicine, Université de Montréal, PO Box 6128 Station Centre-Ville, Montreal, Quebec, Canada H3C 3J7; Department of Computer Science and Operations Research, Faculty of Arts and Sciences, Université de Montréal, PO Box 6128 Station Centre-Ville, Montreal, Quebec, Canada H3C 3J7; Department of Chemistry, Université de Montréal, PO Box 6128 Station Centre-Ville, Montreal, Quebec, Canada H3C 3J7; Division of Hematology, Hôpital Maisonneuve-Rosemont, 5415 de l'Assomption Boulevard, Montreal, Quebec, Canada H1T 2M4.
JournalNat Commun
Year2016
AbstractIn view of recent reports documenting pervasive translation outside of canonical protein-coding sequences, we wished to determine the proportion of major histocompatibility complex (MHC) class I-associated peptides (MAPs) derived from non-canonical reading frames. Here we perform proteogenomic analyses of MAPs eluted from human B cells using high-throughput mass spectrometry to probe the six-frame translation of the B-cell transcriptome. We report that ∼ 10% of MAPs originate from allegedly noncoding genomic sequences or exonic out-of-frame translation. The biogenesis and properties of these 'cryptic MAPs' differ from those of conventional MAPs. Cryptic MAPs come from very short proteins with atypical C termini, and are coded by transcripts bearing long 3'UTRs enriched in destabilizing elements. Relative to conventional MAPs, cryptic MAPs display different MHC class I-binding preferences and harbour more genomic polymorphisms, some of which are immunogenic. Cryptic MAPs increase the complexity of the MAP repertoire and enhance the scope of CD8 T-cell immunosurveillance.
Curation Last Updated2023-08-18 22:30:47
Related Information
Epitopes
Bcell Assays0
Tcell Assays
MHC Ligand Assays