Reference | ||
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Reference Type | Literature | IEDB_Reference:1037971 |
Title | Profiling SARS-CoV-2 HLA-I peptidome reveals T cell epitopes from out-of-frame ORFs. | |
Authors | Shira Weingarten-Gabbay; Susan Klaeger; Siranush Sarkizova; Leah R Pearlman; Da-Yuan Chen; Kathleen M E Gallagher; Matthew R Bauer; Hannah B Taylor; W Augustine Dunn; Christina Tarr; John Sidney; Suzanna Rachimi; Hasahn L Conway; Katelin Katsis; Yuntong Wang; Del Leistritz-Edwards; Melissa R Durkin; Christopher H Tomkins-Tinch; Yaara Finkel; Aharon Nachshon; Matteo Gentili; Keith D Rivera; Isabel P Carulli; Vipheaviny A Chea; Abishek Chandrashekar; Cansu Cimen Bozkus; Mary Carrington; MGH COVID-19 Collection & Processing Team; Nina Bhardwaj; Dan H Barouch; Alessandro Sette; Marcela V Maus; Charles M Rice; Karl R Clauser; Derin B Keskin; Daniel C Pregibon; Nir Hacohen; Steven A Carr; Jennifer G Abelin; Mohsan Saeed; Pardis C Sabeti | |
Affiliations | Broad Institute of MIT and Harvard, Cambridge, MA 02142, USA; Department of Organismic and Evolutionary Biology, Harvard University, Cambridge, MA 02138, USA. Electronic address: shirawg@broadinstitute.org; Broad Institute of MIT and Harvard, Cambridge, MA 02142, USA. Electronic address: sklaeger@broadinstitute.org; Department of Biochemistry, Boston University School of Medicine, Boston, MA, USA; National Emerging Infectious Diseases Laboratories, Boston University, Boston, MA, USA; Cellular Immunotherapy Program and Cancer Center, Massachusetts General Hospital, Charlestown, MA 02129, USA; Harvard Medical School, Boston, MA 02115, USA; Harvard Program in Biological and Biomedical Sciences, Harvard Medical School, Boston, MA 02115, USA; Repertoire Immune Medicines, Cambridge, MA 02139, USA; Center for Infectious Disease and Vaccine Research, La Jolla Institute for Immunology (LJI), La Jolla, CA 92037, USA; Department of Molecular Genetics, Weizmann Institute of Science, Rehovot 76100, Israel; Translational Immunogenomics Laboratory, Dana-Farber Cancer Institute, Boston, MA, USA; Center for Virology and Vaccine Research, Beth Israel Deaconess Medical Center, Boston, MA, USA... | |
Journal | Cell | |
Year | 2021 | |
Abstract | T cell-mediated immunity plays an important role in controlling SARS-CoV-2 infection, but the repertoire of naturally processed and presented viral epitopes on class I human leukocyte antigen (HLA-I) remains uncharacterized. Here, we report the first HLA-I immunopeptidome of SARS-CoV-2 in two cell lines at different times post infection using mass spectrometry. We found HLA-I peptides derived not only from canonical open reading frames (ORFs) but also from internal out-of-frame ORFs in spike and nucleocapsid not captured by current vaccines. Some peptides from out-of-frame ORFs elicited T cell responses in a humanized mouse model and individuals with COVID-19 that exceeded responses to canonical peptides, including some of the strongest epitopes reported to date. Whole-proteome analysis of infected cells revealed that early expressed viral proteins contribute more to HLA-I presentation and immunogenicity. These biological insights, as well as the discovery of out-of-frame ORF epitopes, will facilitate selection of peptides for immune monitoring and vaccine development. | |
Curation Last Updated | 2025-02-11 00:19:02 |
Related Information | |
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Epitopes | |
Bcell Assays | 0 |
Tcell Assays | |
MHC Ligand Assays |